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Analysis of discordant results.
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1) Product Images from "Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes"

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

Journal: Diagnostics

doi: 10.3390/diagnostics12030751

Analysis of discordant results.
Figure Legend Snippet: Analysis of discordant results.

Techniques Used: Diagnostic Assay, Amplification

Example of discordant results. ( a – d ), a micro-metastatic sentinel lymph node that expressed BRAF V600E when analyzed by IHC and was negative with the BRAF Idylla test despite delayed amplification. ( e – g ), a subcutaneous melanoma metastasis considered negative for IHC BRAF V600E when compared to the control. ( g ) (some background noise due to intracytoplasmic pigment), when stained with a red chromogen the interpretation remains equivocal ( h , i ) and positive with the Idylla method. ( a , e ) HES stain ×20; ( b ) Melan A IHC ×20 (Clone A103, Roche Ventana); ( c , f ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana); ( g ) BRAF V600E IHC ×40 external control showing viable tumor cells and melanophages; ( h ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana—Red detection kit); ( i ) BRAF V600E IHC ×40 external control viable tumor cells with red chromogen; ( d , j ) Amplification curves with the Idylla Explore tool.
Figure Legend Snippet: Example of discordant results. ( a – d ), a micro-metastatic sentinel lymph node that expressed BRAF V600E when analyzed by IHC and was negative with the BRAF Idylla test despite delayed amplification. ( e – g ), a subcutaneous melanoma metastasis considered negative for IHC BRAF V600E when compared to the control. ( g ) (some background noise due to intracytoplasmic pigment), when stained with a red chromogen the interpretation remains equivocal ( h , i ) and positive with the Idylla method. ( a , e ) HES stain ×20; ( b ) Melan A IHC ×20 (Clone A103, Roche Ventana); ( c , f ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana); ( g ) BRAF V600E IHC ×40 external control showing viable tumor cells and melanophages; ( h ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana—Red detection kit); ( i ) BRAF V600E IHC ×40 external control viable tumor cells with red chromogen; ( d , j ) Amplification curves with the Idylla Explore tool.

Techniques Used: Amplification, Control, Staining

Overview of studies combining immunohistochemistry and Idylla for BRAF evaluation.
Figure Legend Snippet: Overview of studies combining immunohistochemistry and Idylla for BRAF evaluation.

Techniques Used: Immunohistochemistry, Mutagenesis, Immunostaining

Comparison of Idylla and IHC for the detection of the BRAF V600E mutation in melanomas.
Figure Legend Snippet: Comparison of Idylla and IHC for the detection of the BRAF V600E mutation in melanomas.

Techniques Used: Comparison, Mutagenesis, Expressing, Blocking Assay, Selection

Related Articles

Formalin-fixed Paraffin-Embedded:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Mutagenesis:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Diagnostic Assay:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Amplification:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Control:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Staining:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Immunohistochemistry:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Immunostaining:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Comparison:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Expressing:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Blocking Assay:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Selection:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Sequencing:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Digital PCR:

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology.
Article Snippet: Slides were stained with a BRAF p.V600Edirected antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.

Article Title: BRAF V600E mutational status in bile duct adenomas and hamartomas.
Article Snippet: Ana€ıs Pujals, Paulette Bioulac-Sage, Claire Castain, C ecile Charpy, Elie Serge Zafrani & Julien Calderaro Department of Pathology, Assistance Publique-Hôpitaux de Paris, CHU Henri Mondor, Cr eteil, France, Facult e de M edecine, Universit e Paris-Est Cr eteil, Cr eteil, France, Inserm U955, Equipe 9, Institut Mondor de Recherche Biom edicale, Cr eteil, France, Department of Pathology, CHU de Bordeaux, Pellegrin Hospital, Bordeaux, France, Inserm, UMR-1053, Universit e de Bordeaux, Bordeaux, France, and Inserm U955 Equipe 18, Institut Mondor de Recherche Biom edicale, Cr eteil, France

Article Title: Prospective evaluation of two screening methods for molecular testing of metastatic melanoma: Diagnostic performance of BRAF V600E immunohistochemistry and of a NRAS-BRAF fully automated real-time PCR-based assay
Article Snippet: Slides were stained with anti-BRAF V600E mutant-specific antibody (clone VE1, dilution 1:200, pH9, Eurobio) [ ] .

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes
Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Article Title: BRAF-associated bile duct adenomatosis: a new entity?
Article Snippet: Sir: Bile duct adenomas (BDAs) are benign intrahepatic biliary proliferations consisting of bile ducts arranged in small and tortuous tubules and embedded in a dense fibrous stroma.. Microscopically, tubules are lined by a single layer of cuboidal to columnar cells with nuclei showing neither atypia nor mitoses.. BDAs were formerly considered to be reactive by some authors.

Article Title: BRAF p.V600E-Negative Langerhans Cell Histiocytosis Associated with a Periapical Cyst: A Case Presentation with Broad Review of the Differential Diagnosis and Disease Pathophysiology
Article Snippet: Slides were stained with a BRAF p.V600E-directed antibody (clone VE1, dilution 1:200, pH9, Eurobio), S100, CD1a, Langerin (CD 207) and Ki-67.



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Analysis of discordant results.

Journal: Diagnostics

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

doi: 10.3390/diagnostics12030751

Figure Lengend Snippet: Analysis of discordant results.

Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Techniques: Diagnostic Assay, Amplification

Example of discordant results. ( a – d ), a micro-metastatic sentinel lymph node that expressed BRAF V600E when analyzed by IHC and was negative with the BRAF Idylla test despite delayed amplification. ( e – g ), a subcutaneous melanoma metastasis considered negative for IHC BRAF V600E when compared to the control. ( g ) (some background noise due to intracytoplasmic pigment), when stained with a red chromogen the interpretation remains equivocal ( h , i ) and positive with the Idylla method. ( a , e ) HES stain ×20; ( b ) Melan A IHC ×20 (Clone A103, Roche Ventana); ( c , f ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana); ( g ) BRAF V600E IHC ×40 external control showing viable tumor cells and melanophages; ( h ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana—Red detection kit); ( i ) BRAF V600E IHC ×40 external control viable tumor cells with red chromogen; ( d , j ) Amplification curves with the Idylla Explore tool.

Journal: Diagnostics

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

doi: 10.3390/diagnostics12030751

Figure Lengend Snippet: Example of discordant results. ( a – d ), a micro-metastatic sentinel lymph node that expressed BRAF V600E when analyzed by IHC and was negative with the BRAF Idylla test despite delayed amplification. ( e – g ), a subcutaneous melanoma metastasis considered negative for IHC BRAF V600E when compared to the control. ( g ) (some background noise due to intracytoplasmic pigment), when stained with a red chromogen the interpretation remains equivocal ( h , i ) and positive with the Idylla method. ( a , e ) HES stain ×20; ( b ) Melan A IHC ×20 (Clone A103, Roche Ventana); ( c , f ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana); ( g ) BRAF V600E IHC ×40 external control showing viable tumor cells and melanophages; ( h ) BRAF V600E IHC ×20 (Clone VE1, Roche Ventana—Red detection kit); ( i ) BRAF V600E IHC ×40 external control viable tumor cells with red chromogen; ( d , j ) Amplification curves with the Idylla Explore tool.

Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Techniques: Amplification, Control, Staining

Overview of studies combining immunohistochemistry and Idylla for BRAF evaluation.

Journal: Diagnostics

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

doi: 10.3390/diagnostics12030751

Figure Lengend Snippet: Overview of studies combining immunohistochemistry and Idylla for BRAF evaluation.

Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Techniques: Immunohistochemistry, Mutagenesis, Immunostaining

Comparison of Idylla and IHC for the detection of the BRAF V600E mutation in melanomas.

Journal: Diagnostics

Article Title: Comparison of Two Rapid Assays for the Detection of BRAF V600 Mutations in Metastatic Melanoma including Positive Sentinel Lymph Nodes

doi: 10.3390/diagnostics12030751

Figure Lengend Snippet: Comparison of Idylla and IHC for the detection of the BRAF V600E mutation in melanomas.

Article Snippet: Vallée et al. 2019 [ ] , Melanoma (metastatic and primary) , 65 , Clone VE1 (Eurobio) , BRAF V600E , No , NS , FFPE tissue , Idylla NRAS- BRAF -EGFR S492R Mutation Test , V600 E/E2/D V600 K/R , 89 (overall) , 82,3 , 100 , 100 , 93.

Techniques: Comparison, Mutagenesis, Expressing, Blocking Assay, Selection